Diatoms possess an exotic combination of light-harvesting pigments, Chls a/c and fucoxanthin, making them an interesting platform for studying the photoreceptors of photoinhibition. Light-induced damage to PSII is called photoinhibition, and the damaging reactions of photoinhibition are still under debate. Light-dependent electron transfer is necessary for photosynthesis, but light also damages PSII. Os resultados demonstraram ser o nitrogênio o fator limitante primário para o crescimento do fitoplâncton.Īction Spectrum of Photoinhibition in the Diatom Phaeodactylum tricornutum. As respostas à s diferentes condições ambientais fornecidas as culturas, foram obtidas baseando-se na diferenciação da biomassa em termos de clorofila-a, e na realização da fotossÃntese em termos da assimilação do carbono-14. Foram realizadas 176 ensaios biológicos com o auxÃlio de Phaeodactylum tricornutum, incubada em dez diferentes combinações de meios, além do controle. 45Â✠1' W, na superfÃcie e a 50,0 m de profundidade. The nitrogen out measured in terms of Carbon-14 assimilation and cloropyll concentration, was found to be a primary limiting factor for marine phytoplankton production.Este trabalho teve como finalidade avaliar a qualidade das águas costeiras coletadas num ponto nas proximidades da Ilha Vitória (Lat. Different water enrichements were made by the aseptic addition of several nutrients, at each depth, according to Smayda (1964.
Nutrient experiment using Phaeodactylum tricornutum as an assay organismįull Text Available The growth of Phaeodactylum tricornutum, cultured at 7,000 lux and 25º C, in twelve-day experiments using enriched water collected at the surface and 50.0 m depth from coastal waters offshore of Ubatuba area, was carried out. tricornutum is very promising, although, as discussed, overlooked pitfalls have to be considered. Thus, we confirm that application of the CRISPR/Cas9 system for P. In the end, we identified cells, showing either monoallelic or homo-biallelic targeted mutations. In our experiments, we targeted two different genes, screened for mutations and analyzed mutated diatoms in a three-step process. We constructed an easy-to-handle expression vector for application in the model organism Phaeodactylum tricornutum and tested its capabilities in order to apply CRISPR/Cas9 technology for our purpose. Optimizing CRISPR/Cas9 for the Diatom Phaeodactylum tricornutumĭirectory of Open Access Journals (Sweden)įull Text Available CRISPR/Cas9 is a powerful tool for genome editing. The experiments were carried out at different reaction times (5 and 15 min) and over a wide range. The microalgae Phaeodactylum tricornutum was processed by hydrothermal liquefaction in order to assess the influence of reaction temperature and reaction time on the product and elemental distribution. Sigaard Christensen, Per Peng, Gaël Vogel, Frédéric Adav has appeared on broadcasts such as The Rachel Maddow Show, Anderson Cooper 360, NBC Nightly News, and National Public Radio's Morning Edition, and he has been cited in publications nationwide, including the New York Times, Washington Post, USA Today, Politico, Slate, and Reuters.Hydrothermal Liquefaction of the Microalgae Phaeodactylum tricornutum In addition to his litigation and policy practice, Adav regularly provides expert analysis for television, radio, and print journalism.
He is admitted to the bars of New York and the District of Columbia. Adav has also served as a Special Assistant United States Attorney for the District of Columbia.Īdav is a graduate of New York University School of Law (J.D.), Georgetown University (M.A.L.S.) and the University of Pennsylvania (B.A.). From 2007 to 2012, as acting assistant general counsel and an attorney in the FEC’s Litigation Division, he litigated campaign finance matters, including the landmark cases of Citizens United v.
From 2013 to 2017, as associate general counsel for policy, Adav oversaw all legal recommendations regarding the FEC’s regulations and advisory opinions. Prior to joining CLC, Adav served for more than 10 years in several capacities within the Office of General Counsel of the Federal Election Commission. Adav has conducted dozens of constitutional cases in district courts, courts of appeals and the United States Supreme Court.
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